SysInternals does it again and delivers another impressive tool: RootkitRevealer
RootkitRevealer is an advanced root kit detection utility. It runs on Windows NT
4 and higher and its output lists Registry and file system API discrepancies that
may indicate the presence of a user-mode or kernel-mode rootkit. RootkitRevealer successfully detects all persistent rootkits published at www.rootkit.com, including AFX, Vanquish and HackerDefender (note: RootkitRevealer is not intended to detect memory-based rootkits like Fu that don't survive reboots).
Via [Sysinternals]
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